Southgate returned to the England camp for a meeting with his players, who were surprised when he walked in with his right arm in a sling.
England suffered their first important injury of their 2018 World Cup campaign on Wednesday, but fortunately for their chances of triumphing in Russian Federation, the victim was not a player, but coach Gareth Southgate.
Pick your formation and starting XI - and don't forget to share your team on social media.
Southgate had been in temporary charge from September 27 after Sam Allardyce had resigned and while he has yet to be properly tested, so far he has passed most of the tests which have come his way with flying colours.
England began fully focusing on the Group G clash with Panama on Thursday, when attention on manager Southgate after dislocating a shoulder swiftly switched to his assistant.
Sterling was short of his best in Volgograd and was replaced by Rashford.
Meanwhile, Stones has revealed how City teammate Vincent Kompany took time out to help him improve his game and ensure his place at the World Cup.
This is expected to be England's toughest game before the knockout stages. Stones says they won't change that approach due to their opponents.
"I was very nervous going into that England squad".
Southgate's side must be wary of conceding throw-ins in risky positions as this will be bring the danger of Adolfo Machado into play.
"It is going to be man versus man and we are not going to look at the names on the back of the England jerseys".
Panama lack serious quality but they are experienced, organised and physical.
Kick off is at 3pm local time (1pm United Kingdom time), which is when the mercury is expected to peak.
"All of the managers I worked for had different qualities, different strengths and different weaknesses, but they were all the same in that they were totally single-minded".
"Let's talk about the other night".
"England are a bit more direct than Belgium".
Kane scored twice in the opening 2-1 win over Tunisia and the England captain might see Panama as an opportunity to boost his bid to win the tournament's Golden Boot.