"The Trinidad and Tobago Football Association and his former national team-mates at this time extend deepest condolences to his family members both in the United Kingdom and here in Trinidad and Tobago".
An athletic and composed left-footed player, he was earmarked as one of the country's most promising left backs and represented England at Under-18, Under-20 and Under-21 level.
The English Football League tweeted: "The #EFL is deeply saddened to hear of the passing of Jlloyd Samuel".
Bolton added: 'Rest in peace, Jlloyd.
Samuel made 199 appearances for Villa after signing with the club in 1998, and played 83 times for Bolton between 2007 and 2011.