The lawsuit "alleges that Spotify is using Petty's "Free Fallin", the Doors' "Light My Fire", and tens of thousands of other songs without a license and compensation".
The publisher said it was seeking the maximum allowed $150,000 in damages for copyright damages for each song, meaning an award of at least $1.6 billion, along with the fees of its lawyers.
The music industry has long since been at odds with music streaming and it appears that the battle isn't over yet.
A recent proposed settlement involving rights holders and Spotify in the class action lawsuit Ferrick et al. v. Spotify USA Inc.et al does not adequately compensate Wixen or the songwriters it represents.
Before Spotify launched in the US, the company made deals with major record labels to obtain the appropriate rights to the sound recording copyrights in the songs, the lawsuit states.
In that settlement, Spotify admitted to failing to obtain necessary statutory licenses to reproduce and/or distribute musical compositions on its platform, the lawsuit says.
I've reached out to Spotify and Wixen.
A representative for Spotify declined Variety's request for comment.
The suit goes on to accuse Spotify of outsourcing licensing-obtaining responsibilities to a third party, the Harry Fox Agency, which was "ill-equipped to obtain all of the necessary mechanical licenses".
The complaint also claims that more than 20 percent of Spotify's 30 million songs could be unlicensed. Wixen claims that Spotify has used their copyrighted material without a license.