The 39-year-old Buffon said that Atletico, who would dearly love revenge over Real in the final in Cardiff, might have an edge over the other three teams because they are out of the La Liga title race so can rest players for Europe.
"But I am convinced we will have a derby in the semi-finals".
The semifinals are May 2 & 3, 9 & 10; the draw will be this Friday.
The final is on June 3 at the Millennium Stadium in Wales.
There was no repeat of Barca's spectacular recovery at Camp Nou, having fought back to remove Paris Saint-Germain thanks to a 6-1 second-leg thriller in the last 16, as Lionel Messi, Luis Suarez and Neymar were nullified by the Italian visitors.
Atletico Madrid have been losing finalists in two of the last three seasons, but will look to go one better this year as they chase their first ever triumph in the tournament.
"It's a letdown to be knocked from the Champions League but winning the league relies on winning in the Bernabeu and that's our goal".
At 11am, or shortly afterwards following the usual homilies, we shall find out the draw for the 2017 Champions League semi-finals.
Monaco will tackle Juventus in the other semi-final.
The semi-finals of this season's UEFA Champions League took shape on Wednesday after Juventus and AS Monaco defeated respective quarter-final opponents Barcelona and Borussia Dortmund to advance in the competition.
But while most eyes are on the main competition, there is still all to play for in the Europa League too. The Red Devils are desperate to qualify for the Champions League and winning the Europa League will help them in case they miss out on a top-four finish in the Premier League.