Thursday, 21 June, 2018

Researchers successfully thaw cryopreserved tissue with no damage

Melissa Porter | 04 March, 2017, 02:53

The researchers were also able to successfully wash away the iron oxide nanoparticles from the sample following the warming - although said that further safety testing would be required before the technique could be used in patients.

"This paper takes the first practical step towards making tissue banking a reality", said Caitlin Czajka, an editor at Science Translational Medicine, which published the research on Tuesday. Now, it's a straight shot to cryopreserving full human organs. The process forestalls ice crystal formation, which could damage the tissues, and instead maintains a liquid state of very high viscosity, similar to glass.

Scientists have succeeded in cryogenically freezing and rewarming sections of heart tissue for the first time, in an advance that could pave the way for organs to be stored for months or years. Attempts to rewarm vitrified tissue over the years have invariably resulted in matrix disruption, or the material cracking and crystallizing.

FROZEN organs could be brought back to life safely one day with the aid of nanotechnology, a new study finds. This translates to hundreds of degrees Celsius per minute, and the heat must be uniform.

The so-called "nanowarming" method begins by covering the organ in specially engineered, 50-nanometre wonderballs, made of iron oxide coated with silica, a polymer and a nasty little chemical called trimethoxysilane (the coating helps keep the iron oxide balls dispersed in a solution).

In addition to Bischof, the study authors from the University of Minnesota include postdoctoral researchers Navid Manuchehrabadi, Zhe Gao, Jin Jin Zhang, Hattie Ring, and Qi Shao; graduate student Feng Liu; undergraduate student Michael McDermott; Dentistry Professor Alex Fok; Radiology Professor Michael Garwood; Chemistry Professor Christy Haynes. "I knew we were onto something that could potentially be transformative".

"We will have to actually go to a larger system if we want to move into human organs but there's nothing that precludes us from doing that". "The technology does exist". The next step for researchers is to try the technique on rat and rabbit organs.

Human trials with skin and muscle are planned within 18 months and then the technology could be trialled on parts of a human face and hands.

The radiofrequency coil used in the experiment and a heart sample.

As for bodily cryopreservation, which could allow us to freeze our bodies now and reinvigorate them centuries later, the researchers are less optimistic. Brockbank is confident this method could be perfected in the next decade and open the way for cryopreserved organ banks. Speed and even heating are the keys to preventing this from happening.

Since transplant organs have a very narrow time frame of use and must arrive to the recipients within 48 hours after being harvested, more than 60 percent of donor hearts and lungs fail to make it in time to the patients and end up being discarded.

A group of United States scientists have successfully thawed cryopreserved tissue without damage to the sample, a huge step towards being able to use frozen or preserved tissues in transplantation, according to a new study. This has been identified as a method of the indefinite storage of tissues for transplantation. Consequently, now used thawing methods are not a viable option for long-term cryopreservation of tissue and organs. They then generated heat using an external magnetic field in order to warm the solution uniformly. Larger samples of pig heart tissue too big for today's heating tools also were thawed by the new technology without signs of damage, the researchers reported.

Brockbank and colleagues previously attempted and failed to use microwave warming to generate an even thawing.